Dynamics of field-amplified sample stacking
نویسندگان
چکیده
منابع مشابه
Dynamics of field-amplified sample stacking
Field-amplified sample stacking (FASS) uses conductivity gradients and resulting non-uniform electromigration fluxes to effect concentration increases of analyte ions. For cases where the initial sample concentration is much smaller than the background electrolyte (BGE) concentration, the ideal maximum concentration enhancement is equal to γ , the ratio of conductivity of the sample solution to...
متن کاملNonlinear Effects in Field Amplified Sample Stacking
In field amplified sample stacking (FASS), the sample solution is typically prepared in low conductivity buffer or DI water, so that sample ion concentration can be on the order of the buffer ion concentration. Under these conditions, sample ions can strongly affect conductivity gradients and this leads to nonlinear dispersive effects due to electromigration. We have developed a model to invest...
متن کاملSingle-step Capillary Electrophoresis for Field-amplified Sample Stacking
We developed a novel single-step capillary electrophoresis (SSCE) scheme for field-amplified sample stacking (FASS) by using a hydrophilic material polymethyl methacrylate (PMMA) microchannel chip. The capillary effect was used to introduce liquids and control capillary stop valves in a partial barrier structure of the microchannel. Through the combined action of stop valves and air vents, both...
متن کاملEffect of a low-conductivity zone on field-amplified sample stacking in microchip micellar electrokinetic chromatography.
The effects of a low-conductivity (low-C) zone of an on-line sample preconcentration based on field-amplified sample stacking (FASS) under an acidic condition was investigated in microchip micellar electrokinetic chromatography (MCMEKC). By employing originally fabricated microchips with T- and cross-channel injectors on a single device, partial injection of the low-C zone and large-volume inj...
متن کاملField-amplified sample stacking β-cyclodextrin modified capillary electrophoresis for quantitative determination of diastereomeric saponins.
Successful simultaneous diastereomeric separation and sensitive determination of two pairs of triterpenoidal saponins have been achieved by capillary electrophoresis (CE) using β-cyclodextrin (β-CD) as a stereoselective agent to cooperate with borate complexation. A usual technique for isolation and group separation of saponins was developed as an appropriate purification step prior to the dete...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Fluid Mechanics
سال: 2005
ISSN: 0022-1120,1469-7645
DOI: 10.1017/s0022112005005975